Over the course of this year, I am going to blog about my National Center for Science Education (NCSE) Science Communication Fellowship and share all the wonderful resources that I come across along the way. I am super excited for this opportunity, all of the skills I am going to gain, and the science communication events I am going to participate in. If it was not for twitter (shout out to @NMLaudicina), I would not have known about this opportunity. So I started to wonder how many people could really benefit and thoroughly enjoy this fellowship, that may have never heard of it. So before all of the awesomeness starts, I wanted to use this first blog post to talk a little about NCSE and what exactly the NCSE Science Communication Fellowship is.
NCSE was first established in 1981 to help local communities assure that evolution was accurately being taught in the classroom. They have since taken on climate change as well, as it has been highly disregarded in much of the United States. Their mission states "NCSE promotes and defends accurate and effective science education, because everyone deserves to engage with the evidence." NCSE actively works to promote and support scientifically controversial topics, using a no-conflict approach. For more information on NCSE and the achievements in science that they have helped facilitate, you can click here.
NCSE has three primary focuses. The first is "Flare-Ups". These are very specific incidences where a community needs the support of NCSE to prevent the removal of climate change or evolution from a classroom curriculum. These are unplanned events, that often require instant action. If you have a flare-up in your community, you can contact NCSE here. The second focus is "NCSE Teach". This was the focus that I was most familiar with. This branch of NCSE focuses on supplementing teachers with training and activities for evolution and climate change. They have programs that specifically teach teachers how to teach controversial subjects with no-conflict, they have programs that bring scientist and teachers together for "scientist in the classroom" activities, and they have a plethora of activities for evolution and climate change online that can be downloaded for use in the classroom or for STEM outreach. I highly recommend everyone take advantage of all of the resources available on the NCSE website .
So what is the science communication fellowship? What does it involve? Well I am so glad that you asked! I am just starting out and of course will update you as I go along, but I do have an overview of what is going to occur throughout the year. So the fellowship runs for 12 months. My fellowship started January 1st, 2019 and will run to December 31, 2019. With that being said, it is not a yearly fellowship that runs at a set time. It is based on available funding. I was told that they believe that they will have the next open call in JULY (I will definitely tweet out as soon as I know for sure). The overall purpose of the fellowship is to start a booster club in your area. The booster club allows hands-on learning in the community, with the purpose of increasing science literacy. NCSE had booster clubs nationwide! Throughout the year, the fellowship asks that you meet biweekly through a video conference with the other fellows and the Director of Community Science Education, Dr. Kate Carter. We discuss a series of topics (see above and below), all to prepare us for the launch of our booster club.
We also have to participate in at least 5 community outreach events a semester (spring, summer, and fall)... practice, practice, practice! Lastly, we have to do a video diary series (a vlog)! Anyone that knows me, knows that I am geeking out over the vlog! I am so excited. What is even more exciting is that they sent us a tripod and light, so that it is quality recording. The moment I heard this, the ideas started to flow. I cannot wait to get started on this! Throughout the year we will also survey our community and identify the scientific needs of the community and write an outreach grant. Additionally, it comes with a $9000 stipend with the commitment of 50 hours a month (your PI has to sign off on it too). This opportunity is going to be able to provide me an idea of what doing #scicomm full time would be like. That is a huge advantage verses taking a job and finding out later that it is not right for me.
The flu vaccine kit that was sent for the first semester of outreach.
They also send you quarterly kits, made up of 2 community activities. These are really neat! This first set had a kit on flu vaccines (check out those little pigs!) and another on climate change, specifically on rising tides. I will write more on these kits and share material once I work through them! Whats even cooler, is that the fellowship helps you design your own activity. Your activity will then be sent out to booster clubs across the country! A kit is estimated to reach ~10,000 people... just amazing! If this all sounds as exciting as I think it does, I highly recommend you apply. They take applications from graduate students (masters and Ph.D.), Post-Docs, and adjunct faculty. I would be happy to share my successful application if you want to email me!
Women in Science at Duquesne University (WIS@DU) is an organization of women, from full professors to undergraduates, that strives to encourage and empower women of all ages. The organization holds seminars addressing common issues for women (and men!) such as negotiating, work-life balance, and time management. The organization also offers a mentor network for undergraduates, a place where students can participate in small group discussions on topics of interests and seek advice and guidance. Another large component of WIS@DU is STEM outreach. Due to the generous support of EQT Corporation, WIS@DU has been able to visit several schools in the greater Pittsburgh area. During these events, STEM departments across Duquesne University bring hands-on activities to local schools to help enrich the schools STEM Programs. These events have been a huge success, not only for the students but for the members of WIS@DU as well!
This Fall WIS@DU visited West Greene High School in Greene County, Pennsylvania. This was an all day event where members of WIS@DU spent the morning at the school talking about local abandoned mine drainage (AMD) and then visited a local passive remediation system designed to treat AMD. The day started with a presentation by Dr. Nancy Trun, Associate Professor in the Biological Sciences Department at Duquesne University, on abandoned mine drainage (AMD), how AMD forms, and the devastation that occurs. She talked about how AMD comes in different forms (acidic and circum-neutral) and how there are different ways to treat AMD. Lead by Duquesne University graduate student Michelle Valkanas, the Women in Science (WIS@DU) group (Dr. Nancy Trun, Brianna Ports, Marisa Guido, Joanna Burton, and Mackenzie Martin) then did two in class activities. The first activity was a pH experiment that provided the students the ability to visualize the differences in pH. This activity was done using red cabbage, a natural pH indicator, and a series of household items going from very acidic to basic (lemon juice, floor cleaner, vinegar, baking soda, and antacids). The red cabbage was purple at circum-neutral (pH 7) and changed to red/pink at acidic pH (<4) and blue/green at basic pH (>8).
Details on the red cabbage pH indicator can be found here.
The second activity that WIS@DU did in the classroom was the building of a DIY water filter. This activity was done using only things that could be bought in the store: sand, pebbles, activated carbon, and a coffee filter. The students layered the carbon, sand, and pebbles into a 1 liter water bottle and filtered dirty water through the filter. The students were immersed in this activity, optimizing their filters and troubleshooting when the water was still coming out dirty!
The students at West Greene High School try make their own water filters and brainstorm the most efficient way to construct them.
We then went to visit a passive remediation system. Passive remediation systems are designed to treat AMD. There is a passive system, Maiden Mine, that is in Greene County less than an hour from the school. There we got a tour from Daniel Guy, an environmental scientist from Stream Restoration Inc., who discussed the different components of the passive system and how it works to remediate AMD. He even showed us the opening into the mine and took pH measurements throughout the tour to show the efficiency of the system. The students enjoyed the tour and one even asked Daniel for an internship at Stream Restoration Inc. this summer.
Daniel from Stream Restoration showing the students what bituminous coal looks like!
The opening of the Maiden Mine where you can see the iron hydroxide streamers and algae lining the top of the water (left) and Daniel from Stream Restoration talking to the students about the history of the mine and the technologies being used to restore the watershed (right).
Students hiking across the system on their way to the biotic pond that comes before the wetlands.
Women in Science members (left to right) Michelle Valkanas, Bri Ports, and Marisa Guido.
Science communication. I have been tossing around how to tackle this subject for sometime. I finally decided that I needed to stop thinking about it and just start writing. That is after all how things get done, right? I want to start off by saying that I lOVE science communication, this may make my views slightly biased. However, I still think a lot can be gleaned from what I am going to discuss.
So what is science communication. It is exactly what is in the name. It is the communication of science. In a more specific definition, applying to an academic field, it typically refers to communicating science to the general public (non-experts). It is not limited to science, but really applies to all STEM fields. I also like to use it when talking about STEM outreach at schools. Science communication events can occur pretty much anywhere, but are commonly found at libraries, museums, non-profit events, and at high schools and middle schools.
Women in Science at Duquesne University participating in a science communication event last spring; we presented a workshop called DNA-tastic, at a local Pittsburgh middle school.
So what is the main objective? The main objective for science communication is to increase public awareness. The idea is to make science readily available and accessible. In order for science to be impactful, it needs to be understood. Good science communication takes complex ideas and presents them in a manner that is easy to understand. All ages can benefit from science communication, from young children with a limited STEM program in their school, to adults unaware of current scientific developments. STEM is a major component of all of our futures in some form or another and it is important that we understand what is currently developing and where we are going in the future. Additionally, science communication can raise awareness, in a fun approachable way. This is useful when tackling controversial subjects like global warming and evolution.
There is a lot of fun ways to engage in science communication. I think it depends on what you feel comfortable with. Suzi Spitzer wrote a great blog on the five principles of science communication based on a science communication event held by the National Academy of Sciences she attended last fall. Of these points, my favorite is to "communicate with people, rather than to them"! I think that this is so important. People do not want to be talked at.
Contaminated water demonstration. Three tubes of AMD (left picture) and the first tube after the pH was brought up with sodium hydroxide (right picture)
Conversation is key. People are generally interested in engaging in STEM activities, they enjoy talking and learning about it. What they do not want is to be lectured. That is why I often find a fun activity, an eye catcher, that breaks the ice and gets people talking. For example, I take acidic mine drainage (AMD), that visually is clear, and place it among other forms of AMD that is visibly contaminated. I then ask people if they can identify the most contaminated sample and which one they think is the least contaminated. The clear sample is picked 9/10 times. I then bring up the pH by adding sodium hydroxide and watch everyones face in disbelief. The increase in pH causes all of the contamination to precipitate out (i.e. fall out) of solution. This then opens the door for conversation about AMD and how it is extremely prevalent in Pennsylvania and how you cannot always visually identify AMD.
My concept map from the Science Communication Fellowship workshop at Phipps Conservatory.
Science communication takes practice. As scientist, we are often immersed in our work, living and breathing science ALL THE TIME. This sometimes make it difficult to talk about it in a way that non-experts can understand. That is why, I believe, that it is important to not only engage in science communication (practice makes perfect), but to try to find workshops or fellowships to better train you in science communication. When I first started participating in science communication outreach events, I thought that I was doing a great job. That I was effectively disseminating my science in a way that people could understand. It was not till I participated in a Science Communication Fellowship program at Phipps Conservatory, that I realized that I was still presenting a technical presentation, not a conversation. Dr. Sarah States and Dr. Maria Wheeler-Dubas led a one day workshop, where we did a series of activities that helped to tease apart our science in a way that could be easily understood.
The workshop was divided into 5 topics, all of which had several activities within it. 1. The complexity of exchanging information
It was in this first session that we talked about what we "bring to the communication table". We talked about different types of stakeholder groups and how it is important to understand each's groups motivation, before, engaging in communication. That you needed to know where people were coming from, to be able to connect with them. Stakeholders can be government/local agencies, communities, companies, or an individual. The stakeholders involved depends on the topic and it is important that you think of all those that have a stake in your research so that you are prepared to talk to anyone in an informative manner.
2. Creating a more accessible message
Then we went on to work on the message we were hoping to disseminate. We talked about knowing your audience. That each individual comes to you with previous knowledge and/or opinions. It is important to be aware of this, so you can communicate in the most effective way. Ways to discuss your science thru a story was also discussed. It was emphasized that people connect well to stories. This was a very effective exercise. Lastly, we did an exercise that was called, "What's in a Word". It was a challenging exercise, but probably the most useful thing I did that day (and my favorite!). We were given a worksheet that had three columns (term, meaning, and alternative word) and several blank rows. We were told to fill in terms commonly used in our field and then challenged to find alternatives that would be more easily received. It really made me realize that even when I thought I was removing jargon from my presentation, I was not. I really encourage EVERYONE to try this exercise.
3. Meaningful and impacting science engagement
We then moved on to making a concept map. This allowed us to brainstorm a toolkit that covers all aspects of successfully engaging with public. We also did a role playing game, where we presented our elevator pitches and the others in the group would act like different "stake holders", asking each other questions. It was very useful to be probed with questions. It not only brought to light when I was not effectively portraying my science, but brought up things I did not think of. For example, I was asked in this exercise "what they could do to help". I was so use to presenting my science at academic conferences, it did not occur to me that individuals may want to get involved! How excited I was to go home and find an answer to that question.
4. Sharing your own science story
It was in this section that we worked on our own story. The instructors explained that often the audience is looking to trust you and that it is thru sharing personal stories that trust can be gained. They encouraged us to think of our journey, why did we get into science and how/why are we studying what we do. They also shared a list of writing tips, that could be used to form your elevator pitch and your science story.
5. Tabling and table aesthetics
Lastly they talked about tabling. I was tasked to develop a table event to communicate my science. This is referred to as "tabling". The idea of tabling is that you setup an interactive display that people can interact with, while talking with you, the scientist, about your topic. The display should be visually appeasing and eye-catching. It should have staggered layers, rather than being uniform in height. It should have bright colors and items that can be picked up and played with. Tabling is commonly seen in science communication events.
My table display at Meet a Scientist event at Phipps Conservatory in May 2018.
A close up of my poster board on abandon mine drainage in Pennsylvania.
My table was on abandon mine drainage in Pennsylvania, specifically in southwestern Pennsylvania. I had a poster board that had pictures of local devastation caused by AMD. This allowed people to relate to the problem, as this was occurring in the areas they were familiar with or even lived in. I then had a series of Winogradsky columns of different sizes, from big ones that showed great detail to small ones that were in 50ml conical tubes that could be picked up and analyzed. Winogradsky columns are a great science communication tool because it allows people to see a diverse group of bacteria in a safe manner. It is also a fun DIY activity that people can do at home. I had printed instructions on how to make your own DIY Winogradsky column at home. I have adapted the instructions from here and then attached a great reference image for the kids to identify different kinds of bacteria. I also had the clear water demonstration (explained above), to grab everyone's attention.
Reference image I sent home with DIY Winogradsky Columns. Printed from: www.hhmi.org/biointeractive/poster-winogradsky-column-microbial-evolution-bottle
Once I completed my table display, I brought the display back for Maria to look over and approve my display. She was extremely encouraging and gave great tips and suggestions! (Seriously, Maria is fantastic! I highly recommend following her on twitter, she is always posting useful science communication tips and events). Then I was ready to participate in a Meet a Scientist event. I participated in a small interview with Maria, that was posted on Phipps website promoting the event.
Presenting my science at the Meet a Scientist event, May 2018.
Nothing is better than wearing a lab coat! Meet a Scientist, May 2018.
The Meet a Scientist event was setup in one of the rooms at Phipps Conservatory where patrons could stop and visit my exhibit. It was a really engaging experience that allowed me to interact with not only children, but adults as well. We talked about abandoned mine drainage and how Pennsylvania has over 3,000 miles of contaminated watersheds. I had a list of resources if individuals wanted to know more and contact information for non-profit organizations that were working to restore the environmental systems.
Hands-on is an important aspect to every science communication event.
Me at my table, during Meet a Scientist event at Phipps Conservatory
It was my experience participating in the Science Communication Fellowship that I truly developed a love for science communication. I think that it is so incredibly important. The problem is that, so often, as scientist we are constantly pressured to perform (grants, papers, results.. results.. results..), that we leave no time for service. I believe that it is our responsibility as scientist to effectively communicate our findings to not only colleagues (i.e. experts), but to non-experts as well. That funding agencies and departments should reward individuals effectively finding a balance between scientific success and service (science communication). We should also be encouraging undergraduates, matter of fact, training undergraduates to participate in science communication. I always make sure to have several undergraduates participate in events that I plan. It really benefits the student, giving them practice in communicating the science that they are learning, without becoming overwhelmed with jargon and intricate details. As we continue to progress farther in to the world of robotics, genome editing, and nanoparticles, it is more important than ever to have science communication. People need to not only understand the progressive world we live in, but that everyday people are making revolutionary discoveries and that its not a mad scientist in an ivory tower.
As the semester came to a close, I found myself with mixed emotions. Part of me was relieved, the semester was extremely busy and somewhat chaotic, I will now have so much more time to do research-uninterrupted research (the best kind!). On the other hand, I was going to miss the students and the awesome work that they were doing in class. As we gathered for our annual end of semester party, the excitement the students had for the class continued to show. One student made blue and white cupcakes, signifying the blue/white screen we had performed in class during transformations, and the white cupcakes even had an insert (cookie) inside! Another student had made dirt dessert in the form of our AMD site that we had visited earlier in the semester. These clever, and delicious, desserts not only exemplified their creativity, but their appreciation for a great semester. It was indeed a great semester! In fact, I spent most of the semester bragging about the students. I found myself telling everyone about the great projects that they had developed.
The blue and white cupcakes, signifying the blue/white transformation screen.
The students were given 10 weeks to design and execute a research project involving microbial communities found in abandoned mine drainage. This particular group of students, took the challenge and hit the ground running. Not only was there a nice mix of individual projects, but the students became invested in their projects right from the start. It was amazing to see the growth the students had over the course of the semester, not just growth in laboratory skills and knowledge, but personal growth. I watched individuals who struggled with confidence thrive, introverts come out of their shell, and organizational and planning skills meet optimum levels. It was extremely rewarding to see the students immerse themselves in their science.
Dirt dessert in the form of Lowber Passive Remediation System
So what are these great projects? I am so glad you asked! This semester some of the students isolated manganese and iron bacteria and identified them through Sanger sequencing. Others isolated bacteria from Lowber soil and compared it to soil from Duquesne. They than tested the isolated bacteria from both locations for metal resistance. Two groups looked at antibiotic resistance in bacteria isolated from Lowber compared to lab strains. Though there did not appear to be an increase in antibiotic resistance in bacteria isolated from the AMD, the students did find that lab strain Serratia marcescens exhibited increase resistance when grown in media made with AMD! The students were so excited about their findings that they are continuing their project this summer.
Other students looked at rhizosphere bacteria and the role they play in detoxifying the area around the plant roots. Another project that is being continued by the students next semester in our lab, is a study comparing sulfate reducing bacteria (SRB) found at Lowber (circum-neutral discharge) and Middle Branch (acidic discharge). The students worked this semester on isolating SRBs from both locations, and will identify them and run comparison tests next semester. A student going to grad school in the fall for bioinformatics (Go Nicole!), took her interest in bioinformatics and used 16S sequencing analysis to compare two passive systems to see if their populations were similar. She then focused on iron and sulfur bacteria to see if there was a significant difference between the two.
Despite their differences, there was teamwork even during the playoffs!!
Another student performed a series of stains on the bacteria from across the remediation site. This was right in her wheel house as this is what she is going to grad school for in the fall (YAY!). It was nice to see her incorporate her passions and interests into her independent project. Lastly, a student compared water and bacteria from upstream and downstream of the effluent from the passive system. She is going to law school in the fall, but remained a hard-working, dedicated scientist to the very last minute of class.
Marnie's transition from a scientist to a law student!
At the end of the semester the students presented their independent projects to the class in a powerpoint presentation. It was truly enjoyable watching them present their work. The students went above and beyond this semester to complete their projects and this was evident as they presented their work. I am so proud of all them!
So as the mixed emotions tossed and turned inside of me, I had a moment of clarity. I am a teacher, yes it is what I am contracted to do, but it is more than that. I am invested in the success of my students. I want to see everyone of them succeed and I celebrate their victories and stand by them in their defeats. I wasn't sure if I was going to like teaching, but it has become clear - I do! I am looking forward to the future where I get to do it all over again. Teaching truly is a rewarding experience.
The Superlab IV 2018 students celebrating at the end of the semester party
I remember the first time that I participated in novel research. How excited I was! That excitement quickly turned to pure terror. What do you mean no one has ever answered this question? We do not know the outcome? How do I know I am right? What happens if I am wrong? As I continued, I gained confidence in myself and in my work. It was then that I realized that this was what I wanted to do with the rest of my life, to seek out the unknown and make a difference through scientific discovery. This was my senior year of undergrad at Duquesne University and I was taken a 4 credit lab course, that has been coined "superlab".
Superlab comes in 2 parts, the first semester everyone takes the same course where the students are introduced to a range of lab techniques involving classic microbiology, molecular biology, and protein purification. The second semester comes in several concentrations (physiology, cell biology, and microbiology) where the students are exposed to an in-depth look at a specific topic. I had enrolled in Dr. Nancy Trun's microbiology superlab. This course provides the unique opportunity to engage in novel research, allowing the students to experience science in a way that they typically are not exposed too. They develop hypotheses, design experiments to test these hypotheses, and carry out the designed experiments. Allowing the students to "take the wheel" of their own education, encourages active involvement in the learning experience. They are not just going through the motions, in fact, each individual is creating their own path. I credit this course for the reason that I am in graduate school obtaining a Ph.D. with a concentration in microbiology.
Everybody loves field trip day! Brady, Benita, and Natalie (left to right) on the deck that crosses over the effluent.
I now have the opportunity to be a teaching assistant in the very lab course that inspired me to be where I am today. It provides me the unique perspective that allows me to relate with the students on a more personal level, as I was in their shoes not to long ago. I see the excitement and I see the fear. It is definitely a rollercoaster of emotions (for the students and myself), becoming a scientist is no easy feat! But the students quickly adapt and develop solid hypotheses and execute sound experimental design. It is truly a joy watching the students gain confidence and rise to the challenge, surpassing all of their (and mine) expectations.
Lowber Passive Remediation System.
The class focuses on bioremediation, specifically involving passive remediation systems designed to treat coal mine drainage . The students learn about passive systems, what is known, and what remains unknown. Over the course of the first 6 weeks they form hypotheses and design experiments. Then comes my favorite day of the semester.... (*drum roll please*) FIELD TRIP DAY. We get to take the students to a remediation site (Lowber, see "Field Sites") so that they can collect their own samples. This is always such a fun day, the students really enjoy being in the field. Its funny to hear what the students think the site was going to be like, one student said to me "I thought you climbed deep down into a cave to get to the site". I am sure spelunking was never mentioned in class!
Melissa (left) came ready to go in her boots and fanny pack, while Dania (right) took more of a fashion forward approach, staying pretty in pink.
I am not the only one that gets excited about field trip day, the students do too! Some students came decked out, ready to climb directly into the remediation site, while others were not quite sure what to expect. We started the day with a little background about the site, where Dr. Trun explained how the system worked and what to expect as we moved down through the system. The source pond, where the discharge surfaced from under ground, was a distinct green color. This pond contains high levels of iron and sulfate and has not yet had a chance to precipitate any of the iron. That will quickly change as it moves through the system, turning all of the ponds a bright orange color.
Dr. Trun talks to the students about Lowber and how the passive remediation system works
After the talk, we moved to the end of the system. That way we could work from the effluent, the end of the wetlands, back up to the beginning of the system (where our cars were). Along the way, I noticed that the ponds were orange well into the wetlands. This is not usually the case, but February has had a record amount of rainfall, ~5" alone in the week before we came out. Heavy rainfall increases flow rate, decreasing retention time and does not allow enough time for contaminants (iron especially) to precipitate out. Even the effluent remained tainted with orange.
The yellow boy (orange water) continuing well into the wetlands.
Even after passing through the entire system, the effluent remained orange.
The students really jumped in (some literally) and began to sample. All together we collected 50L of water across all of the 6 settling ponds, the wetlands, and the source pond. The samples will be used to make media, be plated on a variety of selective media types, stained to identify morphology and biochemical properties of bacteria present, and enriched to isolate sulfur, manganese, and iron bacteria. Plant growth and the relationship with rhizosphere bacteria, as well as Sewickley Creek were also sampled for further analysis.
The class disperses with bottles in hand.
Melissa just jumped right in and began to sample the wetlands.
Ali using the sampling pool to get a slurry sample..
Rasha sampling in pond 1. She really took a liking to sampling, jumping at every opportunity to collect slurry.
The system looked so much different from when I was here in January. Everything had thawed, the geese had returned, and biofilms were becoming more prevalent. As we moved from pond to pond, I tried to point out all of the changes that were occurring. Soon, the algae and cyanobacteria will begin to bloom and the wetlands will return to green. Spring is near!
Biofilms forming entering into pond 4 (left) and on the shore of pond 2 (right).
The geese have returned in preparation for spring.
The weather was beautiful and the field trip was a success. The students got to witness the devastation that was happening as a result of abandoned coal mine drainage (AMD). This leads the students to be even more invested in finding possible solutions to treating AMD. It is through this experience, that we (as educators) are able to take what was taught to them in class and materialize it in a way that you can't possibly experience in a classroom.
There is something so beautiful about an early morning following a winter snowfall. There is a sense of purity, for a few moments everything is new and untouched. The air appears cleaner and the world seems still. As Elizabeth Cochran, a masters student in my lab, and I approached Lowber I got a sense of peace, the remediation site had transferred from a place of destruction to a place of beauty overnight. Thats not to say that the devastation that was happening to the land was forgotten (the orange ponds were still prominent), but for one moment the land was once again beautiful, it was breathtaking.
Lowber passive remediation system in Lowber, PA (Winter 2018)
Picture taken in one of the troughs between the ponds
We started at the effluent of the system, the end of the wetlands, and worked our way forward. The wetlands was primarily frozen, but there was still water flowing through a narrow path. It made me wonder if the water was flowing underneath the ice or if water flow was truly limited to a narrow path all year. I think a dye test may be in order this summer to test the water flow from influent to effluent. In the mean time, the water was muddy and not very clear.
Elizabeth taking field measurements in the wetlands (effluent)
Lowber now showing its rendition of Swan Lake
After we sampled 6 liters of water/soil slurry, took field measurements, and freed a cattail from a block of ice, we moved to pond 3. Pond 3 is in the middle of the remediation system and we often study this pond because it serves as a potential inflection point (where contaminants and microbial communities shift). Pond 3 had no ice at all and was six degrees warmer than the effluent. This is to be expected, as the first couple ponds typically don't freeze because the mine discharge is from an underground mine and so it comes into the system fairly warm all year round. There was a few biofilms present as well. Another 6 liters of slurry was collected and Elizabeth found another interesting plant to sample for iron accumulation around the roots.
Pond 3 with no ice
Biofilms found in Pond 3
Here I am taking field measurements in pond 3
Our last stop was pond 1 where another 6 liters of slurry was collected and field measurements were taken. Pond 1 is the first settling pond of the system and was even warmer than pond 3 (~3 degrees warmer). There was a large manganese sheen at the influent of pond 1, suggesting the presence of leptothrix. There was also extensive biofilms growing around the edge of the pond. There was visible bubbles coming from the biofilms. There was a a distinct smell of "rotten eggs", the classic description of sulfide, present at this pond, where the other ponds lacked this lovely smell.
Pond 1 and its massive manganese sheen
Biofilm formation in pond 1, note the bubbles coming up to the surface
As we were walking past the giant mound of iron hydroxide that had been drudged from the ponds (that was now covered in snow), we noticed that there were crystals forming in the snow. Though I cannot explain what was causing this crystallization, it was really cool seeing them laying in the snow! It just confirmed the beauty that had formed at Lowber that cool winter morning.
Crystal formation on top of the iron hydroxide that had been drudged from the ponds
close up of the crystals
The samples collected on this trip will be used for enrichment cultures and lab-based studies to identify bacteria that may serve as bioindicators, as well as bacteria that may be resolubilizing contaminants in the system. mRNA will also be sequenced to track changes in metabolic genes, that could later be used to identify changes/shifts in the system.